Onderzoek lymfoom product verkregen na infusie van piggyBac gemodificeerd CD19 chimeer antigeen receptor van T-cellen
We voerden een Fase I klinische studie uit van van donoren afgeleide CD19-specifieke chimere antigeenreceptor T-cellen (CAR T-cellen) voor B-celmaligniteit die recidiveerde of aanhield na gematchte gerelateerde allogene hemopoëtische stamceltransplantatie. Om de kosten en transgencapaciteitsbeperkingen van traditionele virale vectoren te overwinnen, werden CAR T-cellen geproduceerd met behulp van het piggyBac-transposonsysteem van genetische modificatie. Na CAR-T-celinfusie ontwikkelde één patiënt een geleidelijk groter wordende retroperitoneale tumor als gevolg van een CAR-expressie van CD4+ T-cellymfoom. Screening van andere patiënten leidde tot de detectie van een tweede CAR T-celtumor in thoracale para-aortische lymfeklieren bij een asymptomatische patiënt.
Analyse van het eerste lymfoom toonde een hoog aantal transgenkopieën , maar geen insertie in typische oncogenen. Er waren ook structurele veranderingen zoals een gewijzigd aantal genomische kopieën en puntmutaties die geen verband hielden met de insertieplaatsen. Transcriptoomanalyse toonde aan dat de transgenpromotor de transcriptie van omliggende regio’s opreguleerde ondanks isolatorsequenties die het transgen omringen. Duidelijke globale veranderingen in transcriptie correleerden echter voornamelijk met het aantal genkopieën in plaats van met insertieplaatsen.
Bij beide patiënten vorderde het CAR T-cel-afgeleide lymfoom en één patiënt stierf. We beschrijven de eerste twee gevallen van maligne lymfoom afgeleid van CAR-gen-gemodificeerde T-cellen. Hoewel CAR T-cellen tot nu toe een benijdenswaardige staat van dienst hebben op het gebied van veiligheid, benadrukken onze resultaten de noodzaak van voorzichtigheid en regelmatige follow-up van CAR T-ontvangers, vooral wanneer nieuwe methoden voor genoverdracht worden gebruikt om genetisch gemodificeerde immuuntherapieën te creëren. De proef werd geregistreerd op www.anzctr.org.au als ACTRN12617001579381.
Geheugen T-cellen stamcellen gemodificeerd met een vernieuwde CD30-chimeer antigen receptor vertonen een verhoogd antitumoreffect in Hodgkin lymfoom
Doelstellingen: Adoptieve celtherapie (ACT) met rijpe T-cellen die zijn gemodificeerd met een chimere antigeenreceptor heeft een verbeterd resultaat voor B-celmaligniteiten aangetoond. De toepassing ervan voor andere, zoals Hodgkin-lymfoom, blijft echter een klinische uitdaging. CD30-antigeen, tot expressie gebracht in Hodgkin-lymfoomcellen, is afwezig in de meeste gezonde weefsels en vormt een ideaal doelwit van ACT voor deze ziekte. Desondanks blijft de werkzaamheid van CD30-chimere antigeenreceptor (CAR) T-cellen voor Hodgkin-lymfoom bescheiden. Hier hebben we een nieuwe CD30-CAR T ontwikkeld en getest om de werkzaamheid van CD30-CAR-therapie te verbeteren, met behulp van een targeting-epitoop in het niet-splitsbare deel van de CD30-receptor, en geheugenstam-T-cellen (T SCM) om implantatie, persistentie en antitumoractiviteit te verbeteren.
Methoden: T SCM-achtige culturen werden ex vivo gegenereerd en geëxpandeerd en op dag 1 of two getransduceerd met een lentivirale vector die codeert voor de CD30-CAR. Therapeutische in vivo- experimenten werden uitgevoerd met NSG-muizen die waren geïnjecteerd met L540 (sc) of L428 (iv) en werden behandeld met CD30-CAR T-cellen toen de tumor werd vastgesteld.
Resultaten: CD30-CAR T SCM-achtige cellen gegenereerd en ex vivo geëxpandeerd , ondanks CD30-expressie en broedermoorddoding van CD30 + CAR T-cellen, werden niet aangetast door oplosbaar CD30 en volledig uitgeroeid Hodgkin-lymfoom in vivo , met een hoge persistentie en langdurige immuniteit. Bovendien sterk verrijkte CD30-CAR T SCM-achtige producten verleent een overleving voordeel in vivo , in tegenstelling tot de meer gedifferentieerde CAR T-cellen, met een hogere tumor infiltratie en versterkt antitumoreffect.
Conclusie: Deze studie ondersteunt het gebruik van verfijnde CD30-CAR T-cellen met hoogverrijkte T SCM-achtige producten om de klinische werkzaamheid van CAR T voor Hodgkin-lymfoom te verbeteren.

Human epithelial specific antigen(ESA)ELISA Kit |
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QY-E01682 | Qayee Biotechnology | 96T | EUR 433.2 |
Human epithelial specific antigen,ESA ELISA Kit |
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YLA2715HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human epithelial specific antigen,ESA ELISA Kit |
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YLA2715HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
Anti-Ep-CAM/Epithelial Specific Antigen antibody |
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STJ16100363 | St John's Laboratory | 1 mL | EUR 1041.6 |
Epithelium Specific Antigen antibody |
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10R-E126A | Fitzgerald | 50 ug | EUR 501.6 |
Description: Mouse monoclonal Epithelium Specific Antigen antibody |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx270202 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx232799-100ug | Abbexa | 100 ug | EUR 610.8 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx242428 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx242433 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx224065-100ug | Abbexa | 100 ug | EUR 493.2 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx104775 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx176308 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx172278 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx214622 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx324346 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx331190-100ul | Abbexa | 100 ul | EUR 510 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx327226 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx112318 | Abbexa |
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Epithelial Cell Adhesion Molecule (EpCAM) Antibody |
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abx030033-400ul | Abbexa | 400 ul | EUR 627.6 |
Epithelial Cell Adhesion Molecule (EpCAM) Antibody |
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abx030033-80l | Abbexa | 80 µl | EUR 343.2 |
Epithelial Cell Adhesion Molecule (EpCAM) Antibody |
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abx037446-100ug | Abbexa | 100 ug | EUR 469.2 |
Epithelial Cell Adhesion Molecule (EpCAM) Antibody |
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abx037447-100ug | Abbexa | 100 ug | EUR 469.2 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx125330 | Abbexa |
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Epithelial Cell Adhesion Molecule (EpCAM) Antibody |
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20-abx123431 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx129957 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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20-abx338680 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx430513-200ul | Abbexa | 200 ul | EUR 460.8 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx011925-100ul | Abbexa | 100 ul | EUR 493.2 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody |
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abx159488-100ul | Abbexa | 100 ul | EUR 560.4 |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC942041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF594 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC942041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF594 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC802041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF680 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC802041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF680 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC702041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF770 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC702041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF770 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC882041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF488A conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC882041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF488A conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC812041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF680R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC812041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF680R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC052041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF405M conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC052041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF405M conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC042041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF405S conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC042041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF405S conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC402041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF640R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC402041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF640R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCAP2041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCAP2041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCA2041-250 | Biotium | 250uL | EUR 472.8 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), APC conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCP2041-250 | Biotium | 250uL | EUR 472.8 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), PerCP conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCR2041-250 | Biotium | 250uL | EUR 472.8 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), RPE conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCH2041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCH2041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCB2041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Biotin conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNCB2041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Biotin conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNUB2041-100 | Biotium | 100uL | EUR 316.8 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Concentration: 0.2mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNUB2041-50 | Biotium | 50uL | EUR 486 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), 1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNUB2041-500 | Biotium | 500uL | EUR 615.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), Concentration: 0.2mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC472041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF647 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC472041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF647 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC612041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF660R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC612041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF660R conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC432041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF543 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC432041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF543 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC552041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF555 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC552041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF555 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC682041-100 | Biotium | 100uL | EUR 279.6 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF568 conjugate, Concentration: 0.1mg/mL |
EpCAM / CD326 (Epithelial Marker) (EGP40/2041R) Antibody |
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BNC682041-500 | Biotium | 500uL | EUR 654 |
Description: Primary antibody against EpCAM / CD326 (Epithelial Marker) (EGP40/2041R), CF568 conjugate, Concentration: 0.1mg/mL |
Human Epithelial cell adhesion molecule (EPCAM) |
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1-CSB-EP007717HU | Cusabio |
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Description: Recombinant Human Epithelial cell adhesion molecule(EPCAM),partial expressed in E.coli |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody (PE) |
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20-abx270964 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody (PE) |
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abx140492-100tests | Abbexa | 100 tests | EUR 577.2 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody (APC) |
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20-abx270732 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody (HRP) |
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20-abx335604 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody (FITC) |
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20-abx270500 | Abbexa |
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Epithelial Cell Adhesion Molecule (EPCAM) Antibody (FITC) |
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abx140496-100tests | Abbexa | 100 tests | EUR 510 |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody (FITC) |
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20-abx335605 | Abbexa |
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Human EPCAM/ Epithelial cell adhesion molecule ELISA Kit |
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E0800Hu | Sunlong | 1 Kit | EUR 685.2 |
Mouse EPCAM(Epithelial Cell Adhesion Molecule) ELISA Kit |
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EM1005 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.047 ng/ml |
Epithelial Cell Adhesion Molecule (EPCAM) Antibody (Biotin) |
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20-abx335606 | Abbexa |
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Recombinant Epithelial Cell Adhesion Molecule (EPCAM) |
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4-RPB283Ga01 | Cloud-Clone |
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Description: Recombinant Chicken Epithelial Cell Adhesion Molecule expressed in: E.coli |
Recombinant Epithelial Cell Adhesion Molecule (EPCAM) |
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4-RPB283Hu01 | Cloud-Clone |
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Description: Recombinant Human Epithelial Cell Adhesion Molecule expressed in: E.coli |
Recombinant Epithelial Cell Adhesion Molecule (EPCAM) |
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4-RPB283Po01 | Cloud-Clone |
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Description: Recombinant Pig Epithelial Cell Adhesion Molecule expressed in: E.coli |
Pig Epithelial Cell Adhesion Molecule (EPCAM) Protein |
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20-abx167541 | Abbexa |
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CLIA kit for Mouse EPCAM (Epithelial Cell Adhesion Molecule) |
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E-CL-M0286 | Elabscience Biotech | 1 plate of 96 wells | EUR 700.8 |
Description: A sandwich CLIA kit for quantitative measurement of Mouse EPCAM (Epithelial Cell Adhesion Molecule) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Mouse EPCAM (Epithelial Cell Adhesion Molecule) |
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E-EL-M0470 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
Description: A sandwich ELISA kit for quantitative measurement of Mouse EPCAM (Epithelial Cell Adhesion Molecule) in samples from Serum, Plasma, Cell supernatant |
ELISA kit for Human EPCAM (Epithelial Cell Adhesion Molecule) |
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ELK1968 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Epithelial Cell Adhesion Molecule from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) Protein |
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20-abx652155 | Abbexa |
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Pig Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx361484-96tests | Abbexa | 96 tests | EUR 990 |
Cow Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx516210-96tests | Abbexa | 96 tests | EUR 1093.2 |
Rat Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx516215-96tests | Abbexa | 96 tests | EUR 801.6 |
Chicken Epithelial Cell Adhesion Molecule (EPCAM) Protein |
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20-abx168032 | Abbexa |
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Human Epithelial Cell Adhesion Molecule (EPCAM) CLIA Kit |
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abx196638-96tests | Abbexa | 96 tests | EUR 990 |
Mouse Epithelial Cell Adhesion Molecule (EPCAM) CLIA Kit |
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abx196938-96tests | Abbexa | 96 tests | EUR 990 |
Human Epithelial Cell Adhesion Molecule (EPCAM) CLIA Kit |
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20-abx492586 | Abbexa |
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EPCAM Epithelial Cell Adhesion Molecule Human Recombinant Protein |
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PROTP16422 | BosterBio | Regular: 20ug | EUR 380.4 |
Description: EPCAM Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (24-265 a.a.) and having a molecular mass of 30.1kDa.;EPCAM is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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20-abx151423 | Abbexa |
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Mouse Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx254058-96tests | Abbexa | 96 tests | EUR 848.4 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx350740-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx516212-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx516213-96tests | Abbexa | 96 tests | EUR 848.4 |
Human Epithelial Cell Adhesion Molecule, EPCAM ELISA Kit |
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DEIA244 | Creative Diagnostics | 5 plates | EUR 1612.8 |
Description: The human EPCAM ELISA kit is for the quantitative determination of human EPCAM. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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DLR-EPCAM-Hu-48T | DL Develop | 48T | EUR 597.6 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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DLR-EPCAM-Hu-96T | DL Develop | 96T | EUR 776.4 |
Description: A sandwich quantitative ELISA assay kit for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
Human Epithelial cell adhesion molecule(EPCAM) ELISA kit |
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CSB-EL007717HU-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Human Epithelial cell adhesion molecule (EPCAM) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human Epithelial cell adhesion molecule(EPCAM) ELISA kit |
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1-CSB-EL007717HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Epithelial cell adhesion molecule(EPCAM) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Human Epithelial cell adhesion molecule, EPCAM ELISA KIT |
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ELI-04243h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Epithelial cell adhesion molecule, Epcam ELISA KIT |
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ELI-04247m | Lifescience Market | 96 Tests | EUR 1038 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RDR-EPCAM-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 626.4 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RDR-EPCAM-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 868.8 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RD-EPCAM-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 600 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RD-EPCAM-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 830.4 |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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SEB283Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5402.92 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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SEB283Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 550.13 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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SEB283Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 734.46 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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SEB283Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2945.08 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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4-SEB283Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Epithelial Cell Adhesion Molecule (EPCAM) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RD-EPCAM-Hu-48T | Reddot Biotech | 48T | EUR 432.3 |
Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RD-EPCAM-Hu-96T | Reddot Biotech | 96T | EUR 617.5 |
Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RDR-EPCAM-Hu-48T | Reddot Biotech | 48T | EUR 453.92 |
Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Human Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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RDR-EPCAM-Hu-96T | Reddot Biotech | 96T | EUR 648.38 |
Description: serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
Rabbit Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx363660-96tests | Abbexa | 96 tests | EUR 990 |
Monkey Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx359768-96tests | Abbexa | 96 tests | EUR 990 |
Bovine Epithelial cell adhesion molecule, EPCAM ELISA KIT |
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ELI-04246b | Lifescience Market | 96 Tests | EUR 1113.6 |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig) |
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4-PAB283Po01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM) |
Chicken Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit |
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abx356953-96tests | Abbexa | 96 tests | EUR 990 |
Chicken Epithelial cell adhesion molecule, EPCAM ELISA KIT |
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ELI-04242c | Lifescience Market | 96 Tests | EUR 1113.6 |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human) |
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4-MAB283Hu21 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM) |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Chicken) |
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4-PAB283Ga01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Epithelial Cell Adhesion Molecule (EPCAM) |
ELISA kit for Human Epithelial cell adhesion molecule (EPCAM) |
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KTE62402-48T | Abbkine | 48T | EUR 398.4 |
Description: Quantitative sandwich ELISA for measuring Human Epithelial cell adhesion molecule (EPCAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Epithelial cell adhesion molecule (EPCAM) |
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KTE62402-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
Description: Quantitative sandwich ELISA for measuring Human Epithelial cell adhesion molecule (EPCAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Epithelial cell adhesion molecule (EPCAM) |
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KTE62402-96T | Abbkine | 96T | EUR 646.8 |
Description: Quantitative sandwich ELISA for measuring Human Epithelial cell adhesion molecule (EPCAM) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig), APC |
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4-PAB283Po01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with APC. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig), Cy3 |
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4-PAB283Po01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with Cy3. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig), HRP |
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4-PAB283Po01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with HRP. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig), PE |
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4-PAB283Po01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with PE. |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human), APC |
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4-MAB283Hu21-APC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with APC. |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human), Cy3 |
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4-MAB283Hu21-Cy3 | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with Cy3. |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human), HRP |
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4-MAB283Hu21-HRP | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with HRP. |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human), PE |
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4-MAB283Hu21-PE | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with PE. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Pig), FITC |
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4-PAB283Po01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Pig Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with FITC. |
Epithelial Membrane Antigen (EMA) Antibody |
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abx022470-02mg | Abbexa | 0.2 mg | EUR 1128 |
Epithelial Cell Adhesion Molecule (EPCAM) Monoclonal Antibody (Human), FITC |
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4-MAB283Hu21-FITC | Cloud-Clone |
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Description: A Mouse monoclonal antibody against Human Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with FITC. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Chicken), APC |
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4-PAB283Ga01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with APC. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Chicken), Cy3 |
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4-PAB283Ga01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with Cy3. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Chicken), HRP |
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4-PAB283Ga01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with HRP. |
Epithelial Cell Adhesion Molecule (EPCAM) Polyclonal Antibody (Chicken), PE |
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4-PAB283Ga01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Chicken Epithelial Cell Adhesion Molecule (EPCAM). This antibody is labeled with PE. |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx235427-100ug | Abbexa | 100 ug | EUR 661.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx235428-100ug | Abbexa | 100 ug | EUR 661.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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20-abx133246 | Abbexa |
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Epithelial Membrane Antigen (MUC1) Antibody |
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20-abx121644 | Abbexa |
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Epithelial Membrane Antigen (MUC1) Antibody |
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abx023797-1mg | Abbexa | 1 mg | EUR 1011.6 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx025382-400ul | Abbexa | 400 ul | EUR 627.6 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx025382-80l | Abbexa | 80 µl | EUR 343.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020564-1mg | Abbexa | 1 mg | EUR 1011.6 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020565-1mg | Abbexa | 1 mg | EUR 961.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020566-1mg | Abbexa | 1 mg | EUR 1011.6 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020567-1mg | Abbexa | 1 mg | EUR 1011.6 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020569-1mg | Abbexa | 1 mg | EUR 961.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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abx020571-1mg | Abbexa | 1 mg | EUR 961.2 |
Epithelial Membrane Antigen (MUC1) Antibody |
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20-abx123255 | Abbexa |
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EPCAM ELISA Kit| Mouse Epithelial Cell Adhesion Molecule ELISA K |
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EF013585 | Lifescience Market | 96 Tests | EUR 826.8 |
Chimeer antigeen receptor van T-cellen gericht CD7 bij een variety met een hoog risico T-cel acute lymfoblastische leukemie
Effectieve systemische behandelingen voor recidiverende of refractaire T-cel acute lymfatische leukemie (T-ALL) zijn beperkt. Recente klinische toepassing van chimere antigeenreceptor (CAR) immunotherapie heeft succesvolle beheersing van B-celmaligniteiten door CAR-T-cellen aangetoond; het ontwerpen van CAR’s voor T-ALL blijft echter een uitdaging. Overexpressie van CD7 bij T-celmaligniteiten kan een aantrekkelijk doelwit zijn voor immunotherapie bij T-ALL. Deze studie had tot doel het veilige en effectieve gebruik van autologe CD7-CAR T-cellen (4SCAR7) voor de behandeling van T-ALL met inductiefalen bij een 11-jarige patiënt te beschrijven. Op foundation van het studieprotocol van de Chinese language Youngsters’s Most cancers Group-ALL (CCCG-ALL) werd minimale residuele ziekte (MRD) door flowcytometrie (FC)-analyse gedetecteerd op dag 19 en 46 van de remissie-inductie.
Aan het einde van remissie-inductiechemotherapie bereikte de patiënt morfologische volledige remissie, zij het met MRD 16,13% en RT-PCR van KMT2A-MLLT1- fusie-positief, wat op inductiefalen wees. De cerebrospinale vloeistof (CSF) was negatief voor ontploffing bij de diagnose. CAR-T-therapie en allogene transplantatie werden aanbevolen als de volgende behandelingsopties. CD3 + -lymfocyten werden 18 dagen na de hoge dosis MTX-chemotherapie bij de patiënt verzameld door middel van leukaferese. De 4SCAR7 CD7-targeting CAR-T-cellen werden daarna gegenereerd.
De patiënt kreeg voorafgaand aan de 4SCAR7- infusie lymfodepletie-chemotherapie . Orale toediening van itraconazol en sulfamethoxazol werd uitgevoerd vanaf dag zero na infusie van CAR-T-cellen. De patiënt had geen hypotensie, hypoxie of ernstige biochemische verandering of afwijking, maar had koorts op dag 9. Hoewel graad 1 cytokine-release syndrome (CRS) werd gediagnosticeerd, werd het met succes behandeld met ibuprofen. Anti-CD7 CAR-transgenkopie-aantallen in perifeer bloed werden bepaald door qPCR, die aanvankelijk een effectieve expansie vertoonde, daarna snel daalde en op een laag niveau aanhield. Hoewel de patiënt cytopenie ondervond van dag 14 tot 21, bereikte de patiënt remissie op dag 17. Na volledige remissie ontving de patiënt hematopoëtische stamceltransplantatie (HSCT) en is tot op heden goed hersteld. Over het algemeen suggereerde dit rapport dat 4SCAR7 een veilige en effectieve strategie zou kunnen zijn voor de behandeling van pediatrische patiënten met T-cel-maligniteiten met een hoog risico.
Affected person Reported Outcomes for Most cancers Patiënten met hematologische maligniteiten ondergaan Chimeer Antigeen Receptor T Cell Remedy: een systematische overview
Databases werden doorzocht om onderzoeken te identificeren die in de afgelopen 10 jaar zijn gepubliceerd en die het nut van door de patiënt gerapporteerde uitkomsten (PRO’s) onderzochten bij patiënten die chimere antigeenreceptor (CAR) T-celtherapie kregen bij patiënten met hematologische maligniteiten. Van de 280 data kwamen drie artikelen over 206 patiënten in aanmerking. De gegevens zijn prospectief verzameld op meerdere tijdstippen. De nalevingspercentages waren 70% tot 94%. Er was een omgekeerde relatie tussen vermoeidheid en socialefunctioneren onder volwassenen. De verbetering van de kwaliteit van leven (KvL) en het vermogen om PRO’s te voltooien, waren gekoppeld aan de ziektestatus. Ongeveer 40% van de volwassenen meldde op zijn minst enige cognitieve problemen, met een nadelige invloed op de mentale en fysieke gezondheidstoestand. Bij volwassenen waren geheugenproblemen de meest gemelde cognitieve stoornis.
Depressie ging gepaard met cognitieve problemen . Jongere volwassenen hadden een hoger risico op langdurige slechte geestelijke gezondheid, angst en depressie. Bij pediatrische en adolescente patiënten verbetert de emotionele disfunctie in de loop van de tijd. KvL-status verbeterde in de loop van de tijd; toch veroorzaakten ernstig cytokine-afgiftesyndroom en neurotoxiciteit een vertraagde verbetering. Informatie over de vraag of de PRO’s zijn geïntegreerd in medische dossiers en klinische richtlijnen ontbreekt. Het gebruik van PRO’s bij patiënten met CAR T -celtherapie lijkt haalbaar en informatief. Research met grotere steekproefomvang en het gebruik van gevalideerde PRO-tools op verschillende tijdstippen blijven onvervulde behoeften.